Update data from the database in real time

Can webflow pull data from the database and assign it to global js variables in real time when the data values from the database change?

Nope. Not a current feature on the platform. You may want to consider using an API that does.

No, Webflow itself does not have the capability to directly pull data from a database and assign it to global JavaScript variables in real time when the data values change. Webflow is primarily a visual website builder that focuses on front-end development and provides a content management system (CMS) for managing website content. Try also solving the problem via https://www.devart.com/dac.html

Look into Wized. It may have AJAX subscription hooks that allow your application server to push updates to your active browser clients.